와이즈 토토 앱학과 특별세미나 개최 안내
와이즈 토토 앱학과에서는 특별세미나를 아래와 같이 개최하오니 관심있는 분들의 많은 참석을 바랍니다.
1. 연사: 강준호 박사 (KIST)
2. 일시: 2022. 11. 14(월), 오후 4시 30분
3. 장소: 공학3동 302호 (세미나실)
4. 제목: Physical dynamics of single 와이즈 토토 앱s
Physical properties of 와이즈 토토 앱s, such as mass, volume, and stiffness are valuable indicators of 와이즈 토토 앱 state and function. Numerous 와이즈 토토 앱ular processes including 와이즈 토토 앱 development, differentiation, migration, and disease progression (e.g., cancer metastasis) accompany changes in 와이즈 토토 앱ular physical properties. However, studying the physical dynamics is challenging because both the magnitude and duration of 와이즈 토토 앱ular changes are heterogeneous across the 와이즈 토토 앱 population. In this talk, we present new methods to monitor single-와이즈 토토 앱 stiffness, bioenergetics, and mass over the entire 와이즈 토토 앱 cycle with a temporal resolution of ~1 min.
We utilize the suspended microchannel resonator (SMR), a fluid-filled cantilever capable of measuring the buoyant mass of a 와이즈 토토 앱 by the change of SMR resonant frequency. First, we quantify 와이즈 토토 앱 stiffness via acoustic scattering of waves from a 와이즈 토토 앱 inside the SMR. Through simulations, experiments with hydrogels, and chemical perturbation of 와이즈 토토 앱s, we show that our readout from acoustic scattering measures stiffness1. Second, we monitor the mitochondrial membrane potential of single mouse lymphocytic leukemia L1210 와이즈 토토 앱s and model ATP synthesis dynamics using an electrical circuit model of mitochondria2. We found that mitochondrial ATP synthesis temporarily decreases by approximately 50% during early mitosis. Lastly, we monitored L1210 와이즈 토토 앱 growth during normal proliferation and polyploidization to show that increasing 와이즈 토토 앱 mass by over 100-fold does not limit maximal growth efficiency, indicating exponential growth3. Altogether, these studies revealed physical dynamics and their underlying mechanism throughout the 와이즈 토토 앱 cycle that have not been observed by endpoint assays.
[1] Kang, et al. “Non-invasive monitoring of single-와이즈 토토 앱 mechanics by acoustic scattering”. Nature Methods (2019).
[2] Kang*, Katsikis*, et al. “Monitoring and modeling of lymphocytic leukemia 와이즈 토토 앱 bioenergetics reveals decreased ATP synthesis during 와이즈 토토 앱 division”. Nature Communications (2020).
[3] Mu*, Kang*, et al. “Mass measurements during lymphocytic leukemia 와이즈 토토 앱 polyploidization decouple 와이즈 토토 앱 cycle-and 와이즈 토토 앱 size-dependent growth”. Proceedings of the National Academy of Sciences (2020).
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