야구 토토/BK21 SEMINAR [5.14]
관련링크
본문
Self-Assembly of 야구 토토-Like Structures on Supported Lipid Bilayers
* Speaker : Dr. Kwonmoo Lee (Department of Cell Biology, Harvard Medical School)
* Place : 야구 토토 Seminar Room (Science Bldg, 3-201)
* Date & Time : May 14 (Fri) 3:00 ~ 4:00 pm
Ø Abstract
야구 토토 are finger-like protrusive structures, containing actin bundles. To
probe their mode of assembly we have reconstituted 야구 토토-like structures
(FLSs) by applying frog egg extracts to supported lipid bilayers containing
phosphatidylinositol(4,5)bisphosphate. FLSs assemble parallel actin bundles
from the membrane with known 야구 토토l components localized to the tip and
shaft. The 야구 토토 tip complexes self-organize and are not templated by
preexisting microdomains in the lipid. The BAR domain protein toca-1 recruits
N-WASP then the Arp2/3 complex and actin assembly follow. Elongation proteins,
Diaphanous-related formin, VASP and fascin are recruited later. Although the
Arp2/3 complex is required for FLS initiation, it is not essential for
elongation, which involves formins. We propose a model for 야구 토토 formation
involving an initial clustering of Arp 2/3 complex regulators,
self-assembly of 야구 토토l tip complexes on the membrane, and outgrowth of parallel actin
bundles.
Contact Person : Prof. Wokyung Sung (054-279-2061,wsung@postech.ac.kr)