토토 커뮤니티 순위PHYSICS/ BK21 Colloquium[09
관련링크
본문
"
Toward an Unified Description for Thermodynamics,
Folding Kinetics, and Mutagenesis of Proteins”
* Speaker : Prof. Ik Soo Chang[Pusan National University]
* Place : 와이즈 토토 앱 Seminar Room (Science Bldg, 3-201)
* Date & Time : Oct,와이즈 토토 앱16Fri)와이즈 토토 앱4:00 ~ 5:00 pm
Abstract
A multiscale-heterogeneous protein energy function was developed for the biophysical study of the
thermodynamics, folding kinetics, and mutagenesis of proteins. After hybridizing an와이즈 토토 앱atomic와이즈 토토 앱force-
field into a coarse-grained energy function to account for the specificity of a와이즈 토토 앱sequence와이즈 토토 앱of와이즈 토토 앱amino
acids even with the high structural homology, we constructed an exact free energy landscape of a
given protein and characterized its thermodynamics and folding-unfolding kinetics via와이즈 토토 앱a와이즈 토토 앱Chevron
plot based on solving exactly a master equation, which would enable us to pursue와이즈 토토 앱a와이즈 토토 앱mutagenesis
application. Our approach was applied successfully for resolving a controversy와이즈 토토 앱about와이즈 토토 앱the와이즈 토토 앱folding
mechanism of fast folding proteins.
Recent experiments claiming that BBL protein follows a global downhill folding raised an important
controversy as to the folding mechanism of fast folding proteins. Under the global downhill folding
scenario not only proteins undergo a gradual folding, but also folding events along the continuous
folding pathway could be mapped out from the equilibrium denaturation experiment. Based on the
exact calculation using a free energy landscape, relaxation eigenmodes from와이즈 토토 앱a와이즈 토토 앱master와이즈 토토 앱equation,
and Monte Carlo simulation of an와이즈 토토 앱extended와이즈 토토 앱Munoz-Eaton와이즈 토토 앱model와이즈 토토 앱that와이즈 토토 앱incorporates와이즈 토토 앱multiscale-
heterogeneous pairwise-interactions between amino acids, here we show that the very nature of a
two-state cooperative transition such as a bimodal distribution from an exact free energy landscape
and biphasic relaxation kinetics do manifest in the thermodynamics와이즈 토토 앱and와이즈 토토 앱folding-unfolding와이즈 토토 앱kinetics
of BBL and peripheral subunit-binding domain (PSBD) homologue. Our results provide an unequivocal
resolution to the fundamental controversy related to와이즈 토토 앱the와이즈 토토 앱global와이즈 토토 앱downhill와이즈 토토 앱folding와이즈 토토 앱scheme,와이즈 토토 앱whose
applicability to other proteins should be critically re-examined.
Contact Person : Prof. Ki Bong Lee (054-279-2068, kibong@postech.ac.kr)
"
Toward an Unified Description for Thermodynamics,
Folding Kinetics, and Mutagenesis of Proteins”
* Speaker : Prof. Ik Soo Chang[Pusan National University]
* Place : 와이즈 토토 앱 Seminar Room (Science Bldg, 3-201)
* Date & Time : Oct,와이즈 토토 앱16Fri)와이즈 토토 앱4:00 ~ 5:00 pm
Abstract
A multiscale-heterogeneous protein energy function was developed for the biophysical study of the
thermodynamics, folding kinetics, and mutagenesis of proteins. After hybridizing an와이즈 토토 앱atomic와이즈 토토 앱force-
field into a coarse-grained energy function to account for the specificity of a와이즈 토토 앱sequence와이즈 토토 앱of와이즈 토토 앱amino
acids even with the high structural homology, we constructed an exact free energy landscape of a
given protein and characterized its thermodynamics and folding-unfolding kinetics via와이즈 토토 앱a와이즈 토토 앱Chevron
plot based on solving exactly a master equation, which would enable us to pursue와이즈 토토 앱a와이즈 토토 앱mutagenesis
application. Our approach was applied successfully for resolving a controversy와이즈 토토 앱about와이즈 토토 앱the와이즈 토토 앱folding
mechanism of fast folding proteins.
Recent experiments claiming that BBL protein follows a global downhill folding raised an important
controversy as to the folding mechanism of fast folding proteins. Under the global downhill folding
scenario not only proteins undergo a gradual folding, but also folding events along the continuous
folding pathway could be mapped out from the equilibrium denaturation experiment. Based on the
exact calculation using a free energy landscape, relaxation eigenmodes from와이즈 토토 앱a와이즈 토토 앱master와이즈 토토 앱equation,
and Monte Carlo simulation of an와이즈 토토 앱extended와이즈 토토 앱Munoz-Eaton와이즈 토토 앱model와이즈 토토 앱that와이즈 토토 앱incorporates와이즈 토토 앱multiscale-
heterogeneous pairwise-interactions between amino acids, here we show that the very nature of a
two-state cooperative transition such as a bimodal distribution from an exact free energy landscape
and biphasic relaxation kinetics do manifest in the thermodynamics와이즈 토토 앱and와이즈 토토 앱folding-unfolding와이즈 토토 앱kinetics
of BBL and peripheral subunit-binding domain (PSBD) homologue. Our results provide an unequivocal
resolution to the fundamental controversy related to와이즈 토토 앱the와이즈 토토 앱global와이즈 토토 앱downhill와이즈 토토 앱folding와이즈 토토 앱scheme,와이즈 토토 앱whose
applicability to other proteins should be critically re-examined.
Contact Person : Prof. Ki Bong Lee (054-279-2068, kibong@postech.ac.kr)
"
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